Experimentelles Forschungslabor
Das experimentelle Forschungslabor der Universitätsklinik für Intensivmedizin unterstützt experimentelle und klinische Forschung der Universitätsklinik für Intensivmedizin und anderer Kliniken und führt eigene zellbiologische Untersuchungen durch.
Unser Team verwendet unter anderem folgende Techniken:
- Electron Microscopy/Immune Electron Microscopy
- Light Microscopy
- Enzyme Linked Immunosorbent Assay (ELISA)
- Cell Culture
- SDS Polyacrylamide Gel Electrophoresis (SDS PAGE) and Western Blotting
- Subcellular Fractionation: Isolation of Crude Mitochondria, Pure Mitochondria and Mitochondrial Associated Membranes from Tissues or Cell Cultures
- Determination of Mitochondrial Respiration Rates Using High Resolution Respirometry; Determination of Mitochondrial Membrane Potential
- Determination of Mitochondrial Respiratory Chain Enzyme Activities by Spectrophotometry and Assessment of Citrate Synthase Activity
- Determination of Cellular or Tissue ATP contents
- Isolation of Human White Blood Cells (T cells, B cells, monocytes) Using Magnetic beads